About Dr. Roll
I was born in Baltimore Maryland. My parents decided to relocate to the Midwest so I decided to move with them. I grew up in Kansas City Missouri and then attended Harding University in Search Arkansas where I completed my B.S. degree in chemistry. I completed my Masters degree and PhD degree in biochemistry at the University of Illinois Urbana. I studied the structures of complex carbohydrates on the plasma membrane and inside the cells of chick fibroblasts infected with Rous Sarcoma virus. Graduate studies provided another bonus. While working in the laboratory I met my future wife Peggy and we had the right chemistry. With five children and two grandchildren we are still happily married. We moved to the University of Pittsburgh School of Medicine where I studied the disease of alpha-1-antitrypsin deficiency. I started teaching in the chemistry department at Roberts Wesleyan College after completing my postdoctoral work. I am interested in the synthesis and regulation of topoisomerase enzymes, particularly in Chlamydia bacteria, and the efficient purification of alpha-1-antitrypsin from human serum and calf serum. In addition I am pursuing research projects in forensic science involving DNA identity testing. I have also completed Sabbatical research projects at the Baylor College of Medicine, the University of Rochester School of Medicine, and the Center for Fluorescence Spectroscopy at University of Maryland School of Medicine.
Alpha Chi Sigma Chemical Fraternity
American Scientific Affiliation
American Society for Biochemistry and Molecular Biology
National Association of Advisors for the Health Professions
Federation of American Scientists
Council of Undergraduate Research
Eukaryotic cell differentiation, animal cell membrane and cell surface carbohydrates, glycoprotein synthesis and secretion, viral cell transformation, topoisomerases and cell transformation, topoisomerase genes in Chlamydia.
"Recombinant DNA Technology in the Undergraduate Curriculum," National Science Foundation, College Science Instrumentation Program, Matching Grant $6,000, NSF; $6,000, RWC (1985).
"Cell Biology Laboratory for Advanced Undergraduates," National Science Foundation, College Science Instrumentation Program, Matching Grant $11,000, NSF; $11,000, RWC, (1991).
"Identification of Possible New Chemotherapeutic Targets in Chlamydia," Academic Research Enhancement Award, National Institutes of Health, $93,610, (1996).
“Introducing FT-NMR in the Undergraduate Curriculum,” National Science Foundation, CCLI Adaptation and Implementation program, Matching Grant $27,187.50, (2000).
Coinventors: David Roll, Ignacy Grczynski, Joanna Malicka, Zygmunt Gryczynski and Joseph R. Lakowicz. Patent Disclosure JRL-63 “Metallic Colloid Wavelength-Ratiometric Scattering Sensors”
Publications and Abstracts
Weber, M.J. Hale, R.H., and Roll, D.E., "Role of Protease Activity in Malignant Transformation by Rous Sarcoma Virus,"Proteases and Biological Control, pp. 915-930, Cold Spring Harbor Laboratory, 1975.
Roll, D.E. and Conrad, H.E., "Quantitative Radiochromatographic Analysis of the Major Groups of Carbohydrates in Cultured Animal Cells," Anal. Biochem., 77, 397-412 (1977).
Roll, D.E., Aguanno, J., Coffee, C.J. and Glew, R.H., "Comparison of the Carbohydrate and Amino Acid Composition of Normal and S-Variant Alpha-1-Antitrypsins," Biochem. Biophys. Acta, 532, 171-178 (1978).
Leonard, J., Hale, A., Roll, D., Conrad, H.E. and Weber, M., "Turnover of Cellular Carbohydrates in Normal and Rous Sarcoma Virus-Transformed Cells," Cancer Research, 38, 185-188 (1978).
Roll, D.E., Aguanno, J. and Glew, R.H., "Studies on the Attachment of N-Acetylneuraminic Acid to Glycopeptides from Alpha-1-Antitrypsin," Fed. Proc. 37, Abstract #1828 (1978).
Roll, D.E., Aguanno, J., Coffee, C.J., and Glew, R.H., "Isolation and Characterization of Cyanogen Bromide Derived Glycopeptide Fragments of Alpha-1-Antitrypsin," J. Biol. Chem., 253, 6992-6996 (1978).
Aguanno, J., Roll, D.E., Coffee, C.J., and Glew, R.H., "Studies on the Attachment of N-Acetylneuraminic Acid to Glycopeptides from Alpha-1-Antitrypsin," J. Biol. Chem., 253, 6997-7004 (1978).
Roll, D.E., Weber, M.J., and Conrad, H.E., "Effect of Growth Conditions on the Content of the Major Groups of Carbohydrates in Chick Embryo Fibroblasts," Cancer Research, 39, 2550-2555 (1979).
Durban, E., Roll, D., Beckner, G. and Busch, H., "Purification and Characterization of the Nuclear DNA-Binding Phosphoprotein 110/8.4 Found in Tumor and Fetal Tissues," Fed. Proc. 39, Abstract #1856 (1980).
Durban, E., Roll, D., Beckner, G., and Busch, H., "Purification and Characterization of a Nuclear DNA-binding Phosphoprotein in Fetal and Tumor Tissues," Cancer research, 41, 537-545 (1981).
Roll, D.E., and Glew, R.H., "Isolation and Characterization of Rat Alpha-1-Antitrypsin," J. Biol. Chem. 256, 8190-8196 (1981).
Durban, E., Mills, S., Roll, D. and Busch, H., "Phosphorylation of Purified Hepatoma Topoisomerase I," Biochem. Biophys, Res. Comm., 111, 897-905 (1983).
Rao, M.R.S., Ahr, V.S., Spohn, W.H., Durban, E., Roll, D. and Busch, H., "A Novel Acidic (p14) 19 Kilodalton Nucleolar Phosphoprotein," J. Cell Biology, 97, American Society for Cell Biology Abstract #527, 140a (1983).
McRorie, D.K., Rao, M.R.S., Goldknopf, I.L., Harty, T.P., Roll, D.E., Ahn, V.S., and Busch, H., "Purification and Partial Characterization of a 19KD/pI 4.5 Nucleolar Phosphoprotein," Biochem. Biophys, Res. Comm., 122, 47-55 (1984).
Roll, D. and Durban, E., "The Topoisomerase I Tyrosine Phosphorylation Site and the DNA-Interactive Site," American Society of Biological Chemists. Abstract #1707, (1986).
Roll, D., Software Review of "Simulation of Hemoglobin Function," J. Chem. Ed., 65, A113-114 (1988).
Roll, D., "Recombinant DNA Technology in the Undergraduate Curriculum," abstract and poster submitted to Conference on Gene Splicing sponsored by the American Scientific Affiliation, July, 1988.
Bramucci, M., Roll, D., Cook, R. and Durban, E., "Identification of Consensus Epitope Structures Expressed in Recombinant DNA Libraries," Molecular Immunology, 26, 741-748 (1989).
Roll, D., Murphy, B.J., Laderoute, K.R., Sutherland, R.M. and Smith, H.C., "Oxygen Regulated 80KDa Protein and Glucose Regulated 78KDa Protein Are Identical," Molecular and Cellular Biochemistry, 103, 141-148 (1991).
Daschev, C., Roll, D.E., Bavoil, P.M., "Expression and Translocation of the Chlamydial Major Outer Membrane Protein in Escherichia coli," Microbial Pathogenesis, 15, (1993).
Roll, D., Hsia, R., Bavoil, P.M., "Optimization of Expression and Translocation of the Chlamydial Porin in Escherichia coli." In "Proceedings at the Eighth International Symposium on Human Chlamydial Infection," Orfilia et. al., (eds.), Societo' Editrice Esculapio, Bologna, 263-266 (1994).
Adams, R., Roll, D., "Isolation of a Partial Topoisomerase I Gene Clone from the GPIC strain of Chlamydia Psittaci." Abstract and poster presented at the Ninth International Symposium on Human Chlamydial Infection, June 21-26, 1998, Napa Valley, California.
Geddes, C. D., Parfenov, A. Gryczynski, I., Malicka, J., Roll, D.,and Lakowicz, J. R., “Fractal Silver Structures for Metal-Enhanced Fluorescence: Applications for Ultra-Bright Surface Assays and Lab-on-a-Chip-Based Nanotechnologies,” Journal of Fluorescence, 13(2), 123-128, 2003.
Roll, D., Malicka, J., Gryczynski, I., Gryczynski, Z., and Lakowicz, J. R., “Metallic Colloid Wavelength-Ratiometric Scattering Sensors,” Analytical Chemistry 75(14), 3440-3445 (2003).
Lakowicz, J.R., Malicka, J., Gryczynski, Z., Roll, D., Huang, J., Geddes, C. D., and Gryczynski, I. , “Increased Sensitivity of Fluorescence Detection Using Metallic Nanoparticles,” PharmaGenomics, 3(3), 38-46 (2003).
Geddes, C.D., Parfenov, A., Roll, D., Fang, J., and Lakowicz, J.R., “Electrochemical and Laser Deposition of Silver for Use in Metal-Enhanced Fluorescence,” Langmuir 19(15), 6236-6241 (2003).
Geddes, C.D., Parfenov, A., Roll, D. Gryczynski, I., Malicka, J., Lakowicz, J. R., “Silver Fractal-like Structures for Metal-enhanced Fluorescence: Enhanced Fluorescence Intensities and Increased Probe Photostabilities,” Journal of Fluorescence 13(3), 267-276 (2003).
Geddes, C. D., Roll, D., Parfenov, A., and Lakowicz, J. R., “Noble-Metal Surfaces for use in Metal-Enhanced Fluorescence,” Poster #B705, presented at the 47th Annual Meeting of the Biophysical Society, San Antonio, Texas, March 1-5, 2003.
Geddes, C. D., Parfenov, A., Roldd, D., and Lakowicz, J. R., “Enhanced Photostability of Indocyanine Green on a Roughened Platinum Electrode: Metal-enhanced Fluorescence,” Journal of Fluorescence 13(6), 453-457 (2003).
Geddes C. D., Parfenov, A., Roll, D., and Lakowicz, J. R., “Roughened Silver Electrodes for Use in Metal-enhanced Fluorescence,” Spectrochemica Acta A., 60 (8-9), 1977-1983, (2004).
Zhang, J., Roll, D., Geddes, C. D., and Lakowicz, J. R., “Aggregation of Silver Nanoparticle-Dextran Adducts with Concanavalin A and Competitive Displacement with Glucose,” Journal of Physical Chemistry B, 108 (32), 12210-12214 (2004).